CD16 Mouse anti-Human, eFluor 450, Clone: eBioCB16 (CB16), eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-0168-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15589636

Quantity Price
1 £ 66.65 / 25 tests
EU Stock 2
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Description and Specification

Specification

Quantity 25 Tests
Host Species Murine
Species Reactivity Human
Isotype IgG1
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Regulatory Status RUO
Clone eBioCB16 (CB16)
Antigen CD16
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Concentration 5μL (0.25μg)/test
Applications Flow Cytometry
Conjugate eFluor 450
Monoclonal or Polyclonal Monoclonal
Format Conjugated
Gene Alias Low Affinity IgG Receptor 3, FCGR3a, FCGR3, IGFR11
Primary or Secondary Primary

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.