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CD16, Alexa Fluor 700, clone: eBioCB16 (CB16), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-0168-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15500347

  • £81.61 / 25 tests


Estimated Shipment date
from Supplier 30-05-2018
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Description

Description

Description: The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.Applications Reported: This eBioCB16 (CB16) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioCB16 (CB16) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD16
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O75015, P08637
FCGR3A, FCGR3B, CD16A, CD16B, Fc gamma Receptor III, Fc gamma Receptor 3, RP11-5K23.1, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
eBioCB16 (CB16)
Alexa Fluor 700
FCGR3A
Conjugated
FCGR3A, FCGR3B
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
2214, 2215
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