CD154 (CD40 Ligand), PerCP-eFluor™ 710, clone: 24-31, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-1548-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The 24-31 monoclonal antibody reacts with human CD154, a 39kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.Applications Reported: This 24-31 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripgheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|RP23-153G22.3, CD154, CD40-L, Cd40l, HIGM1, IGM, IMD3, Ly-62, Ly62, T-BAM, TRAP, Tnfsf5, gp39, T-cell antigen Gp39, TNF-related activation protein, tumor necrosis factor ligand superfamily member 5|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.