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CD154 (CD40 Ligand), PE-Cyanine5, clone: 24-31, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-1548-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15517756

  • £94.27 / 25 tests


Estimated Shipment date
from Supplier 27-07-2018
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Description

Description

Description: The 24-31 monoclonal antibody reacts with human CD154, a 39kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.Applications Reported: This 24-31 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD154 (CD40 Ligand)
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P29965
RP23-153G22.3, CD154, CD40-L, Cd40l, HIGM1, IGM, IMD3, Ly-62, Ly62, T-BAM, TRAP, Tnfsf5, gp39, T-cell antigen Gp39, TNF-related activation protein, tumor necrosis factor ligand superfamily member 5
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
CynomolgusþHuman
Flow Cytometry
24-31
PE-Cyanine5
CD40LG
Conjugated
CD40LG
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
959
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