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CD154 (CD40 Ligand), Biotin, clone: MR1, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-1541-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15239749

  • £94.27 / 100µg


Estimated Shipment date
from Supplier 23-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.Applications Reported: The MR1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD154 (CD40 Ligand)
MR1
Biotin
Conjugated
Armenian Hamster
100μg
Store at 2-8°C. Do not freeze.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD40L
IgG
RUO
Primary
Murine
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