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CD153, clone: RM153, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1531-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15227257

  • £143.47 / 500µg


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For Research Use Only.


Description

Description

Description: The RM153 monoclonal antibody reacts with mouse CD153 (CD30 ligand). This 40kDa cell surface receptor, a type II transmembrane protein, is a member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation. Expression of CD153 on T cells does not appear to require costimulation through CD28 and is upregulated via TCR engagement and peaks 24-48 hours after activation. Signaling through CD153 is important in costimulation of pre-activated T cells.Applications Reported: The RM153 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified RM153, cat. 16-1531, in functional assays.).Applications Tested: The RM153 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD153
RM153
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Immunoprecipitation
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD30 Ligand, TNFSF8
IgG2b
RUO
Primary
Murine
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