CD153, clone: RM153, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1531-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The RM153 monoclonal antibody reacts with mouse CD153 (CD30 ligand). This 40kDa cell surface receptor, a type II transmembrane protein, is a member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation. Expression of CD153 on T cells does not appear to require costimulation through CD28 and is upregulated via TCR engagement and peaks 24-48 hours after activation. Signaling through CD153 is important in costimulation of pre-activated T cells.Applications Reported: The RM153 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified RM153, cat. 16-1531, in functional assays.).Applications Tested: The RM153 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD153, CD153 antigen, CD30 antigen ligand, CD30 ligand, CD30-L, CD30L, CD30LG, TNFSF8, tumor necrosis factor (ligand) superfamily member 8, tumor necrosis factor (ligand) superfamily, member 8, tumor necrosis factor ligand 3A, tumor necrosis factor ligand superfamily member 8|
|Flow CytometryþFunctional AssayþImmunohistochemistry (Frozen)þImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.