CD152 (CTLA-4) Monoclonal Antibody (14D3), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15569906	Affymetrix eBioscience 50-1529-42	100 Tests
15559906	Affymetrix eBioscience 50-1529-41	25 Tests

Description

Description: The 14D3 monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling. The 14D3 antibody also recognizes rhesus monkey and has inhibitor activity. Applications Reported: This 14D3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 14D3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.25 μg) per test.

CD152 (CTLA-4) Monoclonal antibody specifically detects CD152 (CTLA-4) in Human, Rhesus Monkey samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD152 (CTLA-4)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P16410
• Gene Symbols: CTLA4
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: CTLA4

• Clone: 14D3
• Applications: Flow Cytometry
• Conjugate: eFluor 660
• Host Species: Mouse
• Target Species: Human, Rhesus Monkey
• Gene ID (Entrez): 1493, 705673
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.