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CD150, PE, clone: 9D1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-1501-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15288679

  • £62.42 / 25µg


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The 9D1 monoclonal antibody reacts with mouse CD150, an ∽70kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers, such as CD150+CD244-CD48-. For this application we recommend the use of antibody clone mShad150 (cat. 12-1502).Applications Reported: This 9D1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD150
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9QUM4
CD150, CDw150, SLAM, IPO-3, signaling lymphocytic activation molecule, SLAMF1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
9D1
PE
Slamf1
Conjugated
Slamf1
IgG1
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
27218
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