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CD15 Monoclonal Antibody (HI98), FITC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£64.50 - £148.00

Specifications

Antigen CD15
Clone HI98
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 2
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Product Code Brand Quantity Price Quantity & Availability  
15526346
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Affymetrix eBioscience
11-0159-41
25 Tests
£64.50
25 tests
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Estimated Shipment: 16-04-2024
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15536346
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Affymetrix eBioscience
11-0159-42
100 Tests
£148.00
100 tests
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Description

Description

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative. Applications Reported: The HI98 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
Specifications

Specifications

CD15
5 μL/Test
Monoclonal
Liquid
RUO
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
stage-specific embryonic antigen 1; CD15; SSEA-1; SSEA1; ELFT; FCT3A; FUC-TIV; FUTIV; LeX
FUT4
Primary
4° C, store in dark, DO NOT FREEZE!
FUT4
HI98
Flow Cytometry
FITC
Mouse
Human
P22083
2526
IgM κ
Affinity chromatography
Antibody
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