CD146, eFluor 450, clone: P1H12, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-1469-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates.The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat.Applications Reported: This P1H12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD146, cell surface glycoprotein MUC18, cell surface glycoprotein P1H12, Gicerin, MCAM, melanoma adhesion molecule, melanoma-associated antigen A32, melanoma-associated antigen MUC18, MUC18, S-endo 1 endothelial-associated antigen|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.