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CD144 (VE-cadherin), clone: eBioBV14 (BV14), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1442-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15217237

  • £157.48 / 500µg


Estimated Shipment
05-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The BV14 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM)+ have the potential to give rise to hematopoietic cells. Cross-blocking experiments suggest that BV14 recognizes a different epitope than another mouse VE-Cadherin monoclonal antibody, BV13.Applications Reported: This eBioBV14 (BV14) antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Intravenous injection of BV14 has been shown to induce a concentration- and time-dependent increase in vascular permeability in the heart and lungs, however BV13 was show to be more potent in this activity. (Please use Functional Grade purified eBioBV13 (BV13), cat. 16-1441, in functional assays.).Applications Tested: This eBioBV14 (BV14) antibody has been tested by western blot analysis of bEnd.3 cell lysates, and can be used in western blotting at a starting concentration of 2 ug/mL.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD144 (VE-Cadherin)
eBioBV14 (BV14)
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Cadherin 5
IgG2b
RUO
Primary
Murine
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