CD144 (VE-cadherin), Functional Grade, clone: eBioBV13 (BV13), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-1441-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Intravenous injection of BV13 has been shown to induce a concentration- and time-dependent increase in vascular permeability in the heart and lungs.Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|Cadherin-5, Vascular endothelial cadherin, VE-cadherin, 7B4 antigen, CD144 antigen, CDH5|
|Store at 2-8°C.|
For Research Use Only.