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CD144 (VE-cadherin), PE-Cyanine7, clone: 16B1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-1449-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15578486

  • £186.79 / 100 tests


EU Stock 1
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Description

Description

Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 16B1 antibody has been pre-titrated and tested by flow cytometric analysis of HUVEC. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD144 (VE-Cadherin)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P33151
Cadherin-5, Vascular endothelial cadherin, VE-cadherin, 7B4 antigen, CD144 antigen, CDH5
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
16B1
PE-Cyanine7
CDH5
Conjugated
CDH5
IgG1
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
1003
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