CD14, FITC, clone: Sa2-8, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 11-0141-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55kDa GPI-linked glycoprotein. CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS. In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form. Sa2-8 has weak antagonistic activity (in NF-kappaB activation or TNF alpha production with LPS stimulation).Applications Reported: The Sa2-8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This Sa2-8 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.