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CD14 Monoclonal Antibody (61D3), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£109.00 - £217.00

Specifications

Antigen CD14
Clone 61D3
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 2
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Product Code Brand Quantity Price Quantity & Availability  
15558356
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Affymetrix eBioscience
25-0149-41
25 Tests
£109.00
25 tests
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15568356
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Affymetrix eBioscience
25-0149-42
100 Tests
£217.00
100 tests
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Description

Description

Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14. Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1.0 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

CD14 Monoclonal antibody specifically detects CD14 in Human samples. It is validated for Flow Cytometry.
Specifications

Specifications

CD14
5 μL/Test
Monoclonal
Liquid
RUO
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
Cd14
Primary
4° C, store in dark, DO NOT FREEZE!
Cd14
61D3
Flow Cytometry
PE-Cyanine7
Mouse
Human
P08571
929
IgG1 κ
Affinity chromatography
Antibody
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