CD14, clone: 61D3, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0149-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 61D3 monoclonal antibody reacts with human CD14, a 53-55kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded human tissue. 61D3 has also been reported for in vitro functional studies. (Please use Functional Grade Purified 61D3, cat. 16-0149, in functional assays, and fluorochrome-conjugated 61D3 is recommended for use in flow cytometry.).Applications Tested: The 61D3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 61D3 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunohistochemistry|
|Store at 2-8°C.|
For Research Use Only.