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CD134 (OX40), PerCP-eFluor™ 710, clone: OX-86, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-1341-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15549186

  • £95.21 / 25µg


Estimated Shipment date
from Supplier 26-07-2018
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Description

Description

Description: The OX-86 monoclonal antibody reacts with mouse CD134, also known as OX40. A member of the TNF receptor superfamily, CD134 is a 50kDa type I membrane glycoprotein expressed by activated mouse T lymphocytes. Rat CD134 was initially identified as an activation marker only on activated CD4+ T cells. In contrast, mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. The interaction of CD134 with CD252 (OX40 ligand) has been implicated in T cell-dependent humoral responses, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4+ T cell compartment.Applications Reported: This OX-86 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OX-86 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD134 (OX40)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P47741
cluster designation 134, TNFRSF4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
OX-86
PerCP-eFluor 710
Tnfrsf4
Conjugated
Tnfrsf4
IgG1, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
22163
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