CD134 (OX40), FITC, clone: ACT35 (ACT-35), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-1347-42

Product Code. 15506496

Description

Description: The ACT35 monoclonal antibody reacts with human CD134, also known as OX-40. A member of the TNF receptor superfamily, CD134/OX-40 is a 50kDa type I membrane glycoprotein expressed by activated T lymphocytes. The interaction of CD134 with OX-40L has been implicated in T cell-dependent humoral response, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool and regulation of tolerance in the CD4^+ T cell compartment.Applications Reported: This ACT35 (ACT-35) antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections.Applications Tested: This ACT35 (ACT-35) antibody has been pre-titrated and tested by flow cytometric analysis of 3-day PHA-activated human normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD134
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P43489
• Gene Alias: cluster designation 134, TNFRSF4
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow CytometryþImmunohistochemistryþImmunohistochemistry (Frozen)
• Clone: ACT35 (ACT-35)
• Conjugate: FITC
• Gene: TNFRSF4
• Format: Conjugated
• Gene Symbols: TNFRSF4
• Isotype: IgG1
• Quantity: 100 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 7293

For Research Use Only.