CD133 (Prominin-1) Monoclonal Antibody (EMK08), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-1339-41

Product Code. 15516886

Description

Description: The EMK08 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites; the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others. The binding of the EMK08 antibody does not block the binding of another anti-human CD133 antibody, TMP4 (cat. 12-1338) indicating that they recognize distinct epitopes. Applications Reported: This EMK08 antibody has been reported for use in flow cytometric analysis. Applications Tested: This EMK08 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. The CD133 (also named AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. It is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. It has been shown to be expressed in cancers, including some leukemias and brain tumours, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver.

Specifications

• Antigen: CD133 (Prominin-1)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: O43490
• Gene Symbols: PROM1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8842
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: EMK08
• Conjugate: PE
• Gene: PROM1
• Gene Alias: AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.