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CD127, PE-Cyanine7, clone: eBioSB/199 (SB/199), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-1273-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15508476

  • £68.21 / 25µg


Estimated Shipment date
from Supplier 04-06-2018
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Description

Description

Description: The SB/199 monoclonal antibody reacts with mouse CD127, the high affinity a subunit of the mouse IL-7 receptor. IL-7 receptor a chain is expressed by immature B cells in the bone marrow, double-negative (CD4-/CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+/CD8+) thymocytes. In the periphery, mature T cells express low levels of CD127. Binding of SB/199 blocks the binding of A7R34, another antibody which recognizes mouse CD127.Applications Reported: This eBioSB/199 (SB/199) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioSB/199 (SB/199) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD127
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P16872
CD127, IL-7Ralpha, MGC107557
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBioSB/199 (SB/199)
PE-Cyanine7
Il7r
Conjugated
Il7r
IgG2b, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
16197
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