CD127, clone: A7R34, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1271-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. It has also been reported in blocking of IL-7 binding in functional studies. (Please use Functional Grade purified A7R34, cat. 16-1271, in functional assays.).Applications Tested: The A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD127, IL-7Ralpha, MGC107557|
|Flow CytometryþFunctional AssayþImmunohistochemistry (Frozen)þImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.