CD127 Mouse anti-Human, eFluor 660, Clone: eBioRDR5, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 50-1278-42

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15529906

Quantity Price
1 £ 182.82 / 100 tests
Estimated Shipment date
from Supplier 03-04-2017
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Description and Specification

Specification

Gene Alias Interleukin-7 Receptor alpha
Concentration 5μL (0.06μg)/test
Regulatory Status RUO
Monoclonal or Polyclonal Monoclonal
Conjugate eFluor 660
Host Species Mouse
Applications Flow Cytometry
Primary or Secondary Primary
Antigen CD127
Format Conjugated
Species Reactivity Human
Quantity 100 tests
Clone eBioRDR5
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Isotype IgG1
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.

Interestingly, recently it has been demonstrated that CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While the co-expression of CD4 and CD25 has become widely used as an indicator of T regs, this method of identification may also include cells without suppressive activity. It has clearly been shown that CD4+CD25+ cells that have down-regulated the expression of CD127 are significantly more highly-enriched for the regulatory T population, as defined by expression of the T reg-specific transcription factor Foxp3 and the suppressive activity of these cells, in vitro.
Binding of the eBioRDR5 monoclonal antibody to PBMCs is blocked by pre-incubation of the cells with recombinant human IL-7 (cat. 14-8079).