CD126, PE-Cyanine7, clone: D7715A7, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 25-1261-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody D7715A7 recognizes mouse CD126, the IL-6 receptor alpha chain, a member of the Ig superfamily. CD126 can heterodimerize with CD130 (gp130) to form the high affinity IL-6 receptor. Once IL-6 is bound, signaling from the CD130 subunit is initiated and the receptor complex is internalized. The IL-6 receptor is also reported to exist as a soluble version, which bound to IL-6 acts in trans to signal. Expression is found on T lymphocytes in the periphery and during development in the thymus is restricted to SP populations, unlike gp130 which is expressed in all thymocytes. Additional expression has been shown on activated B lymphocytes, macrophages, granulocytes and CD34+ hematopoietic progenitor cells. The monoclonal antibody D7715A7 binding can be blocked addition of ligand (IL-6).Applications Reported: This D7715A7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This D7715A7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Interleukin-6 Receptor alpha|
For Research Use Only.