CD126 Monoclonal Antibody (D7715A7), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-1261-82

Product Code. 15598466

Description

Description: The monoclonal antibody D7715A7 recognizes mouse CD126, the IL-6 receptor alpha chain, a member of the Ig superfamily. CD126 can heterodimerize with CD130 (gp130) to form the high affinity IL-6 receptor. Once IL-6 is bound, signaling from the CD130 subunit is initiated and the receptor complex is internalized. The IL-6 receptor is also reported to exist as a soluble version, which bound to IL-6 acts in trans to signal. Expression is found on T lymphocytes in the periphery and during development in the thymus is restricted to SP populations, unlike gp130 which is expressed in all thymocytes. Additional expression has been shown on activated B lymphocytes, macrophages, granulocytes and CD34+ hematopoietic progenitor cells. The monoclonal antibody D7715A7 binding can be blocked addition of ligand (IL-6). Applications Reported: This D7715A7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This D7715A7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

CD126 Monoclonal antibody specifically detects CD126 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD126
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P22272
• Gene Symbols: Il6ra
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16194
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: D7715A7
• Conjugate: PE-Cyanine7
• Gene: Il6ra
• Gene Alias: CD126; CD126 antigen; gp80; IL-6 Receptor; il-6 receptor alpha; IL-6 receptor alpha chain; IL-6 receptor subunit alpha; IL6Q; IL6R; IL-6R; IL-6R 1; IL-6R subunit alpha; IL6R1; IL-6R-1; Il6ra; IL-6RA; IL-6R-alpha; IL6RQ; ILN; Interleukin; interleukin 6 receptor; interleukin 6 receptor, alpha; interleukin-6 receptor subunit alpha; Membrane glycoprotein 80; MGC104991
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b

For Research Use Only.