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CD123, FITC, clone: 5B11, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 11-1231-81
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The 5B11 monoclonal antibody reacts with mouse CD123, the alpha chain of the IL-3 receptor. This 60-70kDa transmembrane protein binds to IL-3 with low affinity by itself and when associated with either CD131 (common beta chain) or AIC2A (IL-3beta) binds IL-3 with high affinity. CD123 does not transduce any intracellular signals upon binding IL-3 and requires the beta chain for this function.Applications Reported: This 5B11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5B11 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspension. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
CD123 | |
Monoclonal | |
0.5mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P26952 | |
CD123, CDw123, SUT-1 | |
Rat | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Murine |
Flow Cytometry | |
5B11 | |
FITC | |
Il3ra | |
Conjugated | |
Il3ra | |
IgG2a, kappa | |
50μg | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Primary | |
16188 |
For Research Use Only.