CD123, FITC, clone: 5B11, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-1231-81

Product Code. 15228179

Description

Description: The 5B11 monoclonal antibody reacts with mouse CD123, the alpha chain of the IL-3 receptor. This 60-70kDa transmembrane protein binds to IL-3 with low affinity by itself and when associated with either CD131 (common beta chain) or AIC2A (IL-3beta) binds IL-3 with high affinity. CD123 does not transduce any intracellular signals upon binding IL-3 and requires the beta chain for this function.Applications Reported: This 5B11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5B11 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspension. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD123
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P26952
• Gene Alias: CD123, CDw123, SUT-1
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: 5B11
• Conjugate: FITC
• Gene: Il3ra
• Format: Conjugated
• Gene Symbols: Il3ra
• Isotype: IgG2a, kappa
• Quantity: 50μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 16188

For Research Use Only.