CD123 Mouse anti-Human, PerCP-Cyanine5.5, Clone: 6H6, eBioscience™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15559026	Affymetrix eBioscience 45-1239-41	25 Tests
15569026	Affymetrix eBioscience 45-1239-42	100 Tests

Description

Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6H6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CDw131) subunit. IL-3Ra is responsible for the transmission of signal of Interleukin-3. IL-3Ra and IL-3Rb chain heterodimerization is induced by Interleukin-3, which is required for receptor activation but not high affinity binding. IL-3Ra is also reported that IL-3Ra subunits are overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells and are thus potential targets for novel therapeutic agents.

Specifications

• Antigen: CD123
• Target Species: Human
• Isotype: IgG1, kappa
• Monoclonal or Polyclonal: Monoclonal
• Format: Liquid
• Type: Antibody
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!

• Clone: 6H6
• Conjugate: PerCP-Cyanine5.5
• Applications: Flow Cytometry
• Primary or Secondary: Primary
• Classification: Monoclonal
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Regulatory Status: RUO

For Research Use Only.