CD123 Monoclonal Antibody (6H6), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15578466	Affymetrix eBioscience 25-1239-42	100 Tests

Description

Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6H6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

CD123 Monoclonal antibody specifically detects CD123 in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD123
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P26951
• Gene Symbols: IL3RA
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: IL3RA

• Clone: 6H6
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3563
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.