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CD122, PerCP-eFluor™ 710, clone: TU27, eBioscience™

Mouse Monoclonal Antibody

£79.25 - £193.00

Specifications

Antigen CD122
Clone TU27
Target Species Human
Conjugate PerCP-eFluor 710
Isotype IgG1, kappa, kappa
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Product Code Brand Quantity Price Quantity & Availability  
15569176
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Affymetrix eBioscience
46-1228-41
25 Tests £79.25
25 tests View alternative products
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Estimated Shipment
01-12-2020
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15579176
View Documents
Affymetrix eBioscience
46-1228-42
100 Tests £193.00
100 tests View alternative products
Save Up to 
»
Estimated Shipment
01-12-2020
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: This TU27 monoclonal antibody reacts with human CD122, which is also known as interleukin-2 receptor beta (IL-2R beta). This receptor is expressed on NK cells, activated T cells, some B cells, monocytes, and T cell leukemias. CD122 can also be detected during early NK cell commitment. IL-2R beta associates with the IL-2R alpha (CD25) or IL-15R alpha and common gamma chain (CD132) to form the high affinity IL-2 and IL-15 receptors, respectively. By itself, this membrane receptor can bind IL-2, but with intermediate affinity. The binding of IL-2 results in internalization of the cytokine, tyrosine phosphorylation of IL-2Rbeta, and activation of signaling cascades such as the JAK/STAT pathway. Applications Reported: This TU27 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TU27 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD122
Human
IgG1, kappa, kappa
Monoclonal
Liquid
Antibody
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
TU27
PerCP-eFluor 710
Flow Cytometry
Primary
Monoclonal
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.