CD120b (TNF Receptor II), clone: TR75-54, eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1203-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The TR75-54 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor II (TNFRII, TNFR-p80, TNFRSF1B, CD120b). TNFRII is expressed in a variety of cell types and strongly expressed on stimulated T and B lymphocytes. TNFRII is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release.Applications Reported: This TR75-54 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and ELISA.Applications Tested: The TR75-54 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse TNFRII in combination with the biotin TR75-32 (cat. 13-1204) antibody for detection and recombinant mouse TNFRII as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|CD120b (TNF Receptor II)|
|Store at 2-8°C.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|TNFR2p80, TNF RII, TNFRII|
For Research Use Only.