missing translation for 'onlineSavingsMsg'
Learn More

CD11b, eVolve 605, clone: M1/70, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 83-0112-41

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15500747

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaM-beta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1/70 antibody has been pre-titrated and tested by flow cytometric analysis of mouse bone marrow cells. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Specifications

Specifications

CD11b
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P05555
CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, Integrin alpha M
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
M1/70
eVolve 605
Itgam
Conjugated
Itgam
IgG2b, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
Primary
16409
Videos

For Research Use Only.