CD11b, eFluor 660, clone: M1/70, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-0112-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaM-beta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The M1/70 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than or equal to 5 ug/mL. The M1/70 antibody is not recommended for immunohistochemistry of formalin-fixed paraffin embedded tissue. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, Integrin alpha M|
|Flow CytometryþImmunohistochemistry (Frozen)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.