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CD11b, eFluor 660, clone: M1/70, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-0112-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341850

  • £134.69 / 100µg


EU Stock 4
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Description

Description

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaM-beta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The M1/70 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than or equal to 5 ug/mL. The M1/70 antibody is not recommended for immunohistochemistry of formalin-fixed paraffin embedded tissue. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD11b
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P05555
CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, Integrin alpha M
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
M1/70
eFluor 660
Itgam
Liquid
Itgam
IgG2b, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16409
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