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CD11b, Functional Grade, clone: M1/70, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0112-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15258377

  • £169.45 / 500µg


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: The M1/70 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections. The M1/70 antibody is not recommended for immunohistochemistry of formalin-fixed paraffin embedded tissue. M1/70 has also been reported in in vitro blocking of CD11b function.Applications Tested: The M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes or bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD11b
M1/70
aqueous buffer, no sodium azide
Integrin alpha M, ITGAM, Mac-1 alpha (Mac1A), Complement Receptor 3 alpha (CR3A)
IgG2b
RUO
Primary
Murine
Immunohistochemistry (Frozen)
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
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