CD11b, Biotin, clone: M1/70, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 13-0112-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. The M1/70 clone is not recommended for immunohistochemical staining of formalin-fixed paraffin embedded tissue.Applications Tested: The M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes or bone marrow cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Integrin alpha M, ITGAM, Mac-1 alpha (Mac1A), Complement Receptor 3 alpha (CR3A)|
For Research Use Only.