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CD11b, PE-Cyanine7, clone: ICRF44, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0118-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15518356

  • £92.18 / 25 tests


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.Applications Reported: This ICRF44 antibody has been reported for use in flow cytometric analysis.Applications Tested: This ICRF44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD11b
ICRF44
PE-Cyanine7
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry
5μL (1.0μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Integrin alpha M, ITGAM, Mac-1 alpha (Mac1A), Complement Receptor 3 alpha (CR3A)
IgG1
RUO
Primary
Rhesus
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