CD11b Mouse anti-Human, FITC, Clone: CBRM1/5, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 11-0113-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15546336

Quantity Price
1 £ 60.53 / 25 tests
EU Stock 1
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Description and Specification

Specification

Applications Flow Cytometry
Quantity 25 tests
Conjugate FITC
Antigen CD11b
Primary or Secondary Primary
Regulatory Status RUO
Monoclonal or Polyclonal Monoclonal
Host Species Mouse
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Clone CBRM1/5
Isotype IgG1
Concentration 5μL (1μg)/test
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Species Reactivity Human
Gene Alias Integrin alpha M, Mac-1 alpha, Complement Receptor 3 alpha (CR3A)
Format Conjugated

The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the α chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.