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CD11b (activation epitope), Alexa Fluor 700, clone: CBRM1/5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-0113-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15550337

  • £195.05 / 100 tests


EU Stock 3
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Description

Description

Description: The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.Applications Reported: This CBRM1/5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CBRM1/5 antibody has been pre-titrated and tested by flow cytometric analysis of resting and activated human peripheral blood leukocytes. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD11b
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P11215
CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, Integrin alpha M
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
CBRM1/5
Alexa Fluor 700
ITGAM
Conjugated
ITGAM
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3684
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