CD11b (activation epitope) Mouse anti-Human, PerCP-eFluor 710, Clone: CBRM1/5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0113-41

Product Code. 15539076

Disclaimers:

For Research Use Only.

Description

The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the \alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.

Specifications

• Antigen: CD11b (activation epitope)
• Clone: CBRM1/5
• Conjugate: PerCP-eFluor 710
• Format: Conjugated
• Host Species: Murine
• Quantity: 25 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Concentration: 5μL (0.125μg)/test
• Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Gene Alias: Integrin alpha M, Mac-1 alpha, Complement Receptor 3 alpha (CR3A)
• Isotype: IgG1
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human