CD11b (activation epitope) Mouse anti-Human, APC, Clone: CBRM1/5, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-0113-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15527876

Quantity Price
1 £ 84.99 / 25 tests
EU Stock 2
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Description and Specification

Specification

Isotype IgG1
Quantity 25 tests
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Conjugate APC
Gene Alias Integrin alpha M, Mac-1 alpha, Complement Receptor 3 alpha (CR3A)
Species Reactivity Human
Monoclonal or Polyclonal Monoclonal
Applications Flow Cytometry
Regulatory Status RUO
Format Conjugated
Antigen CD11b (activation epitope)
Concentration 5μL (0.5μg)/test
Host Species Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Clone CBRM1/5

The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the α chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.