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CD11a (LFA-1alpha), Functional Grade, clone: M17/4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0111-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15218377

  • £94.27 / 100µg


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Disclaimers:

For Research Use Only.


Description

Description

Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis. M17/4 has also been reported in blocking of CD11a function.Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD11a
M17/4
aqueous buffer, no sodium azide
Integrin alpha L, Lymphocyte Function-Associated Antigen
IgG2a
RUO
Primary
Murine
Flow Cytometry
Unconjugated
Purified
Rat
100μg
Store at 2-8°C.
Monoclonal
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