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CD117 (c-Kit) Mouse anti-Human, Super Bright 436, Clone: 104D2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 62-1178-42

Additional Details : Weight : 0.01000kg

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Product Code. 15953404

  • £245.00 / Pack of 100
Estimated Shipment: 13-12-2021

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Description

Description

Description: The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. Applications Reported: This 104D2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions.

Please refer to the datasheet for Super Bright Staining Buffer for more information. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.
Specifications

Specifications

CD117 (c-Kit)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P10721
C-Kit; CD117; KIT; PBT; SCFR
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
104D2
Super Bright 436
KIT
Liquid
KIT
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3815
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SDS
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Certificates
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