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CD117 (c-Kit), Functional Grade, clone: ACK2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-1172-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15288537

  • £49.60 / 50µg


Estimated Shipment
02-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays. (Fluorochrome-conjugated ACK2 is recommended for use in flow cytometric analysis.).Applications Tested: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD117
ACK2
Unconjugated
Purified
Rat
50μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
SCFR, Steel Factor Receptor
IgG2b
RUO
Primary
Murine
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