CD117 (c-Kit) Monoclonal Antibody (ACK2), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-1172-81

Product Code. 15288537

Description

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody. Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays. (Fluorochrome-conjugated ACK2 is recommended for use in flow cytometric analysis.). Applications Tested: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.

001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1&2 signaling pathway.

Specifications

• Antigen: CD117 (c-Kit)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P05532
• Gene Symbols: Kit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16590
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay
• Clone: ACK2
• Conjugate: Functional Grade
• Gene: Kit
• Gene Alias: C-Kit, CD117, PBT, SCFR, mast/stem cell growth factor receptor Kit, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, soluble KIT variant 1, tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein, ckit
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.