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CD117 (c-Kit), clone: ACK2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1172-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15207187

  • £42.19 / 50µg


Estimated Shipment
23-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported for use in functional assays. (Please use Functional Grade purified ACK2, cat. 16-1172, in functional assays.) (Fluorochrome-conjugated ACK2 is recommended for use in flow cytometric analysis.).Applications Tested: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD117 (c-kit)
ACK2
Unconjugated
Purified
Rat
50μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
SCFR, Steel Factor Receptor
IgG2b
RUO
Primary
Murine
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