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CD117 (c-Kit), PerCP-eFluor™ 710, clone: 104D2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-1178-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15559176

  • £225.28 / 100 tests


Estimated Shipment date
from Supplier 20-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.Applications Reported: This 104D2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD117 (c-kit)
104D2
PerCP-eFluor 710
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
SCFR, Steel Factor Receptor
IgG1
RUO
Primary
Human
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