missing translation for 'onlineSavingsMsg'
Learn More

CD115 (c-fms) Rat anti-Mouse, APC-eFluor™ 780, Clone: AFS98, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-1152-80

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15539566

  • £88.75 / 25µg

Estimated Shipment: 25-10-2021

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage. Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse bone marrow-derived macrophages. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat.

00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid maligcy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction.
Specifications

Specifications

CD115 (c-fms)
Monoclonal
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P09581
C-FMS, CD115, CSFR, FIM2, FMS
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
AFS98
APC-eFluor™ 780
Csf1r
Liquid
Csf1r
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12978
Videos
SDS
Documents
Certificates

For Research Use Only.