missing translation for 'onlineSavingsMsg'
Learn More

CD115 (c-fms), APC-eFluor 780, clone: AFS98, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-1152-82

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15549566

  • £189.69 / 100µg


Estimated Shipment date
from Supplier 29-06-2018
Add to basket

Description

Description

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse bone marrow-derived macrophages. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD115 (c-fms)
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
P09581
C-FMS, CD115, CSFR, FIM2, FMS
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
AFS98
APC-eFluor 780
Csf1r
Conjugated
Csf1r
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
12978
Videos

For Research Use Only.