CD107b (LAMP-2), eFluor 660, clone: eBioH4B4 (H4B4), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-1078-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease.Applications Reported: This eBioH4B4 (H4B4) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBioH4B4 (H4B4) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|RP23-193O17.2, CD107b, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, Mac3|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.