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CD107a (LAMP-1), eFluor 450, clone: eBioH4A3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-1079-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15559706

  • £189.69 / 100 tests


EU Stock 3
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Description

Description

Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD107a (LAMP-1)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P11279
CD107a, LAMPA, LGP120, CD107 antigen-like family member A, LAMP-1, lysosome-associated membrane glycoprotein 1, lysosome-associated membrane protein 1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
eBioH4A3
eFluor 450
LAMP1
Conjugated
LAMP1
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3916
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