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CD106 (VCAM-1), eFluor 660, clone: 429, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-1061-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15321880

  • £134.57 / 100µg


EU Stock 2
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Description

Description

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4 beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.Applications Reported: This 429 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD106 (VCAM-1)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P29533
CD106, DKFZp779G2333, INCAM-100, MGC99561, CD106 antigen, vascular cell adhesion protein 1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
429
eFluor 660
Vcam1
Conjugated
Vcam1
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
22329
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