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CD106 (VCAM-1), eFluor 450, clone: 429, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 48-1061-80
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.Applications Reported: This 429 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
CD106 (VCAM-1) | |
Monoclonal | |
0.2mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P29533 | |
CD106, DKFZp779G2333, INCAM-100, MGC99561, CD106 antigen, vascular cell adhesion protein 1 | |
Rat | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Murine |
Flow Cytometry | |
429 | |
eFluor 450 | |
Vcam1 | |
Conjugated | |
Vcam1 | |
IgG2a, kappa | |
25μg | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Primary | |
22329 |
For Research Use Only.