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CD106 (VCAM-1), Functional Grade, clone: 429, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-1061-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15248537

  • £49.99 / 50Āµg


Estimated Shipment
06-07-2018
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Description

Description

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.Applications Reported: The 429 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CD106 in functional studies.Applications Tested: The 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD106
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P29533
CD106, DKFZp779G2333, INCAM-100, MGC99561, CD106 antigen, vascular cell adhesion protein 1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryĆ¾Functional Assay
429
Functional Grade
Vcam1
Purified
Vcam1
IgG2a, kappa
50μg
Store at 2-8°C.
Primary
22329
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